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Planar Lipid Bilayer Workstation

Methods IBC, Einsle/Friedrich/Andrade

Planar Lipid Bilayer Workstation Model: Warner Planar Lipid Bilayer Workstation
Unit and Room: Biochemistry, 10th floor, R.1002
Responsible: Dr. Susana Andrade
Electrophysiology

 Further information: http://portal.uni-freiburg.de/biochemie/equipment
Short Description:

The Planar Lipid Bilayer Workstation allows for pico- or nano-scale charge current measurements across an artifical lipid membrane through reconstituted, ion-conducting single channels. Currents are measured via silver electrodes, digitized and amplified. The entire setup is encased in a Faraday cage for the shielding from electromagnetic and mechanical interference and contains mechanisms for stirring and changing solutions, signal processing and data analysis.

 Picture of the Equipment

Planar Lipid Bilayer Workstation
 
Available Experiments/Techniques:

Painiting of lipid bilayers, reconstitution of membrane proteins;
current measurements; voltage clamp experiments

Special Equipment:

Faraday cage, active vibration-isolation table, membrane support (cups and chambers), dual-capacity stirrer, low electric noise lamp, microscope, perfusion equipment, amplifier, signal filter, osciloscope and analogue-to-digital signal converter.
Measurements on the equipment are currently done by:

Students after extensive training
Trained scientific service personal
Recent Publications, where this instrument was
important (citation):

 

 
Typical problems that may be solved with this instrument:

- measurement of electric currents across lipid membranes.
- determination of ion transport rates through membrane proteins
- measurement of transport activity dependent on lipid membrane composition

 

pdficon.gif PLB-Workstation (this page as pdf file)
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