Stopped-Flow Spectrophotometer
Methods IBC, Einsle/Friedrich/Andrade
| Stopped-Flow Spectrophotometer | Model: | Applied Photophysics SX-20 Stopped-Flow Spectrophotometer |
| Unit and Room: | Biochemistry, 10th floor, R.1002 | |
| Responsible: | Dr. Susana Andrade | |
| Fast Kinetics | Further information: | http://portal.uni-freiburg.de/biochemie/equipment |
| Short Description: The SX20 is used to study transient and pre-steady-state kinetics of fast, liquid-phase chemical and biochemical reactions initiated by the rapid mixing and stopping (stopped-flow) of the reactants. A spectroscopic probe (absorbance or fluorescence) is employed to follow the course of the reaction by recording changes in the amplitude of the spectroscopic signal as a function of time. A typical upper limit to the reaction rates that can be measured with stopped-flow is ~2000s-1 in standard configuration; with smaller volume cells, rates in excess of 3000s-1 can be measured. | Picture of the Equipment
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| Available Experiments/Techniques: Pre-steady-state reaction kinetics are measured through fast mixing of reactants by high-pressure Stopped-Flow. Dead time of the device can be optimized to about 0.5 ms and the reaction is monitored in a 2 x 5 x 10 mm³ cuvette. Two- or four-syringe (aging line) rapid mixing | ||
Special Equipment: | ||
| Measurements on the equipment are currently done by: | Students after extensive training | |
| Recent Publications, where this instrument was important (citation): |
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| Typical problems that may be solved with this instrument: | - Pre steady-state kinetics of chemical and enzymatic reactions | |
Stopped-Flow Spectrophotometer (this page as pdf file)