Protein Crystallization and Imaging
Methods IBC, Einsle/Friedrich/Andrade
| High-Throughput Protein Crystallization and Imaging | Model: | Rigaku Phoenix RE, Alchemist II, Minstrel HT UV, Gallery 700, Douglas Instruments Oryx Nano |
| Unit and Room: | Biochemistry, 9th floor, R. 910 | |
| Responsible: | Dr. Stefan Gerhardt | |
| Protein Crystallography | Further information: | http://portal.uni-freiburg.de/xray/equipment |
| Short Description: Protein crystallization generally involves sparse matrix screening for suitable crystallization conditions in a large number of individual experiments on SBS-format multiwell plates. The setup consists of nanoliter dispensers (Oryx Nano, Phoenix RE) for experiment setup and a temperature-controlle incubation system with automated, scheduled imaging (Minstrel/Gallery). Crystallization screens can be designed on-screen and passed on to a liquid handling system (Alchemist II) for production. External collborators can monitor their crystallization experiments through a web interface. | Picture of the Equipment
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| Available Experiments/Techniques: Crystallization experiments by hanging or sitting drop vapour diffusion or microbatch are prepared using 100-300 nl of sample per drop and are incubated at 4°C or 20°C. Fine screens can be designed around initial hits and pipetted automatically. | ||
Special Equipment: | ||
| Measurements on the equipment are currently done by: | Students after extensive training | |
| Recent Publications, where this instrument was important (citation): | BMC Biochemistry 8:13.
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| Typical problems that may be solved with this instrument: | - Semi-automated screening for crystallization conditions. | |
High-Throughput Protein Crystallization and Imaging (this page as pdf file)