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Polymerase Chain Reaction (PCR)

Methods IBC, Einsle/Friedrich/Andrade

Polymerase Chain Reaction (PCR) Model: Various Thermocyclers & Gradient Thermocyclers
Unit and Room: Biochemistry, 9th floor, R. 903
Biochemistry, 10th floor, R.1008
Responsible: Dr. Stefan Gerhardt
Molecular Biology

Further information: http://portal.uni-freiburg.de/xray/equipment
Short Description:

Thermocyclers for exponential amplification of DNA fragments by temperature cycling through denaturation, annealing and elongation phases.

Picture of the Equipment

Polymerase Chain Reaction (PCR)
 

Available Experiments/Techniques:

Polymerase chain reactions and linear amplifications (single primer) of polynucleotides using the various protocols established for different applications.

Special Equipment:

Gradient Block, Heated Lid.

Measurements on the equipment are currently done by:

Students after Introduction
Students after extensive training
Trained scientific service personal

Recent Publications, where this instrument was
important (citation):

 

 

Typical problems that may be solved with this instrument:

- exponential amplification of DNA fragments from a template
- site-directed mutagenesis of DNA fragments.
- DNA sequencing reactions.

 

pdficon.gif Polymerase Chain Reaction (PCR) (this page as pdf file)

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