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Isothermal Titration Calorimetry (ITC)

Methods IBC, Einsle/Friedrich/Andrade

Isothermal Titration Calorimetry (ITC)	Model:	GE Healthcare / Microcal VP-ITC and ITC200 microcalorimeters
	Unit and Room:	Biochemistry,9th floor, R.910
	Responsible:	Dr. Stefan Gerhardt
Thermal Analysis, Calorimetry	Further information:	http://portal.uni-freiburg.de/xray/equipment
Short Description: The ITC unit directly measures heat evolved or absorbed in liquid samples as a result of mixing precise amounts of reactants. A spinning syringe is utilized for injecting and subsequent mixing of reactants. Spin rates are user selectable. The normal operating range is 2°C to 80°C.		Picture of the Equipment
Available Experiments/Techniques: Protein/ligand and protein/protein titrations for the determination of thermodynmic parameters
Special Equipment: Two devices are available, VP-ITC with a sample volume of 1.4 mL and ITC200 with a sample volume of 200 µL. Sensitivity of the larger machine is slightly superior.
Measurements on the equipment are currently done by:		Students after extensive training Trained scientific service personal
Recent Publications, where this instrument was important (citation):		BMC Biochemistry 8:13.
Typical problems that may be solved with this instrument:		- Quantitation of molecular interactions. - Determination of reaction enthalpies and entropies, binding constants and stoichiometries. - Determination of affinities for ligand screening in drug discovery / lead optimization processes. - Direct measurements of reaction kinetics by enthalpy change.