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High Performance Liquid Chromatography (HPLC)

Methods IAAC, Röhr / Kurz

High performance liquid chromatography (HPLC) Model: Merck-LaChrome-HPLC
Unit and Room: Chemie II, Room 035
Responsible: S. Zuelsdorf
Chromatography Further information: instrument handbook

Short Description:

Quantitative analysis of the organic components in a complex compound mixture by separation through distribution between a stationary phase and a mobile liquid phase.

Picture of the Equipment

High performance liquid chromatography (HPLC)
 

Available Experiments/Techniques:

Separation with monochromatic UV/VIS detection; manual sample injection;
PC control for automatic/manual integration and calibration 

Special Equipment:

Luna 10 µ, C18(2), 250 x 4,6 mm and some other columns

Measurements on the equipment are currently done by: Students after introduction
 
Recent Publications, where this instrument was
important (citation):


 
Typical problems that may be solved with this instrument: quantification of organic components with UV/VIS absorption in a mixture, e.g. coffein in coffee, tea, medications.

 

pdficon.gif High performance liquid chromatography (this page as a pdf file)

 

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